![]() Optical Development in the Zebrafish Eye Lens. The Transparent Lens and Cornea in the Mouse and Zebra Fish Eye. Investigation of Barrier Characteristics in the Hyaloid-Retinal Vessel of Zebrafish. Cell Fate and Differentiation of the Developing Ocular Lens. The Zebrafish Cornea: Structure and Development. Early Retinal Development in the Zebrafish, Danio rerio: Light and Electron Microscopic Analyses. Use of Zebrafish in Drug Discovery Toxicology. The Zebrafish Eye-A Paradigm for Investigating Human Ocular Genetics. Toward a Better Understanding of Human Eye Disease: Insights from the zebrafish, Danio rerio. The authors declare no conflict of interest. The biochemical events led to retinal damage similar to cataract. (2021) studied the involvement of guanylate cyclase activator 1C ( guca1c) in the pathophysiology of primary congenital glaucoma through the generation of a guca1c knocked out zebrafish, which presented cell apoptosis and gliosis, with the Müller cells intensifying the expression of glial fibrillary acidic protein. With loss-of-function techniques in zebrafish (CRISPR and morpholino), several zebrafish models have been reported to recapitulate the phenotypes of patients with ARS attributable to the foxc1 gene mutations (reviewed by. The most striking feature of Axenfeld–Rieger Syndrome (ARS) is developmental abnormalities in the anterior segment of the eye, leading to an increased risk of early-onset glaucoma. Skarie & Link (2009) reported that a mutant zebrafish line with silenced forkhead box C1 ( foxc1) presented altered hyaloid vasculature and arteriovenous defects, as well as ocular hemorrhages and increased vascular permeability, indicative of the disruption of basement membrane integrity, which is related to the glaucoma development process. Finally, the zebrafish model for the study of the pathologies of the visual system complements certain deficiencies in experimental models of mammals since the regeneration of the zebrafish retina is a valuable tool for the study of degenerative processes and the discovery of new drugs and therapies. The pathogenesis of ocular infections, autoimmune diseases, or aging can also be assessed in zebrafish larvae, and the preserved cellular and molecular immune mechanisms can be assessed. Several approaches allow the evaluation of local pathological processes derived from systemic disorders, such as chemical exposure to produce retinal hypoxia or glucose exposure to produce hyperglycemia, mimicking retinopathy of prematurity or diabetic retinopathy, respectively. It is possible to model ocular disorders in zebrafish, as well as inherited retinal diseases or congenital or acquired malformations. The zebrafish genomic database supports genetic mapping studies as well as gene editing, both of which are useful in the ophthalmological field. The zebrafish retina has the same layered structure and cell types with similar metabolic and phototransduction support as humans, and is functional 72 h after fertilization, allowing tests of visual function to be performed. Despite the obvious morphological differences in the visual system, zebrafish share a similar architecture and components of the same embryonic origin as humans.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |